| 质粒名称: | pX330_SpCas9_USP7 Exon 3 gRNA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 131257 |
| 存储实验室: | Roger Woodgate |
|
载体骨架基本信息 |
|
| 载体名称: | pX330-U6-Chimeric_BB-Cbh-hSpCas9 |
| 载体出品公司: | Feng Zhang (Addgene plasmid # 42230) |
| 空载体大小: | 8506 bp |
| 完整质粒大小: | 8509 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | humanised S. pyogenes Cas9 nuclease,hSpCas9 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pX330_SpCas9_USP7 Exon 3 gRNA was a gift from Roger Woodgate (Addgene plasmid # 131257 ; http://n2t.net/addgene:131257 ; RRID:Addgene_131257) |
| 参考文献部分: | DNA polymerase ι interacts with both the TRAF-like and UBL1-2 domains of USP7. Ashton NW, Valles GJ, Jaiswal N, Bezsonova I, Woodgate R. Journal of Molecular Biology. 2020. 10.1016/j.jmb.2020.166733 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pX330_SpCas9_USP7 Exon 3 gRNA 8503 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA
targeting exon 3 of USP7.
ACCESSION .
VERSION .
KEYWORDS pX330_SpCas9_USP7 Exon 3 gRNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8503)
AUTHORS Ashton NW, Valles GJ, Jaiswal N, Bezsonova I, Woodgate R
TITLE DNA polymerase iota interacts with both the TRAF-like and UBL1-2
domains of USP7
JOURNAL Journal of Molecular Biology. 2020.
REFERENCE 2 (bases 1 to 8503)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8503
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..241
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 1..21
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 172..191
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 271..346
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 443..728
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
intron 1003..1230
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1242..1251
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1251..1316
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1323..1343
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1368..5468
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5469..5516
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
primer_bind complement(5544..5561)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5550..5757
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 5766..5906
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5766..5895
/label=AAV2 ITR
rep_origin 5981..6436
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6068..6087)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6278..6299
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6453..6472)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6572..6594
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6632..6650)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6718..6822
/gene="bla"
/label=AmpR promoter
CDS 6823..7683
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7041..7060)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7854..8442
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8343..8362
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
241 cgaaacaccg agaccacacc aaaaaagcgt gttttagagc tagaaatagc aagttaaaat
301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
361 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
421 gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac
481 cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac
541 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
601 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc
661 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
721 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
781 cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg
841 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
901 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
961 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
1021 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
1081 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg
1141 agcaagaggt aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg
1201 gagcacctgc ctgaaatcac tttttttcag gttggaccgg tgccaccatg gactataagg
1261 accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac gataagatgg
1321 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac aagaagtaca
1381 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
1441 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
1501 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
1561 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
1621 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
1681 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
1741 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
1801 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
1861 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
1921 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
1981 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
2041 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
2101 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
2161 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
2221 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
2281 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
2341 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
2401 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
2461 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
2521 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
2581 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
2641 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
2701 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
2761 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
2821 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
2881 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
2941 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
3001 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
3061 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
3121 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
3181 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
3241 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
3301 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
3361 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
3421 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
3481 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
3541 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
3601 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
3661 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
3721 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
3781 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
3841 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
3901 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
3961 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
4021 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
4081 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
4141 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
4201 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
4261 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
4321 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
4381 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
4441 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
4501 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
4561 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
4621 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
4681 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
4741 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
4801 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
4861 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
4921 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
4981 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
5041 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
5101 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
5161 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
5221 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
5281 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
5341 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
5401 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
5461 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagtaag
5521 aattcctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
5581 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
5641 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
5701 ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggagcg
5761 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
5821 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
5881 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
5941 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
6001 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
6061 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
6121 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
6181 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
6241 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
6301 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
6361 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
6421 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
6481 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
6541 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
6601 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
6661 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
6721 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
6781 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
6841 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
6901 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
6961 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
7021 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
7081 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
7141 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
7201 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
7261 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
7321 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
7381 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
7441 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
7501 tggtttattg ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca
7561 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
7621 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
7681 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
7741 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
7801 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
7861 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
7921 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
7981 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
8041 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
8101 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
8161 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
8221 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
8281 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
8341 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
8401 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
8461 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
